Acute Oral Toxicity (LD50) of Crude Exopolysaccharide from Rhodotorula minuta BIOTECH 2178 in ICR Mice

Mary Ann Jilly R. Ramirez, Amelita C. Estacio, Francisco B. Elegado, Ludito V. Ramirez


Acute toxicity study on lyophilized exopolysaccharide (EPS) produced from Rhodotorula minuta BIOTECH 2178 EPS was evaluated using laboratory mice. In this study, 20 male ICR mice were divided into four (4) groups: Group I received 0.5 ml saline solution; Group II was treated with 50mg EPS/kg body weight; Group III was treated with 225mg EPS/kg body weight; and Group IV was treated with 400 mg EPS/kg body weight. Blood samples were collected via orbital sinus for hematological analysis. Body weight, feed and water consumption were recorded daily. Liver and kidney were collected for histopathological analysis after 15 days administration of EPS. Blood chemistry analysis showed that mice fed with crude EPS had comparable BUN, creatinine and ALT values with that of the control. Liver histopathological analysis of the control and treated group showed normal hepatic architecture with intact hepatic cells, nucleus, sinusoidal spaces and a central vein. Histopathological analysis of the kidney showed normal renal structure of cortex, indicating a normal architecture of renal glomeruli, proximal convoluted tubule and distal convoluted tubules. Moreover, the tubules showed a relatively regular distinct lumen of both control and treated groups. Thus, crude EPS from R. minuta BIOTECH 2178 is neither hepatotoxic nor nephrotoxic and likely to be safe for human consumption. Moreover, its safety property lays a good foundation for application of EPS in food and pharmaceutical industries.


Histopathological analysis; Creatinine; Hepatotoxic; Nephrotoxic

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JSET 002


Babicek K, Echovaa I, Simon RR, Harwood M, Cox DJ. (2007). Toxicological assessment of a particulate yeast (1/3 1/6)Beta-D glucan in rats. Food and Chemical Toxicology 45:1719–1730.

Benwahhoud M, Jouad H, Eddouks M, Lyoussi B. (2001). Hypoglycemic

effect of Suaeda fruticosa in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 76, 35–38

Chalasani N, Clark WS, Wilcox CM. (1997). Blood urea nitrogen to creatinine concentration in gastrointestinal bleeding: American Journal Gastroenterology.92:1796–9.

Cheng KC, Demirci A, Catchmark JM, Puri VM. (2011). Effects of initial ammonium ion concentration on pullulan production by Aureobasidium pullulans and its modeling. Journal Food Engineering. 103:115- 1

Cho DH, Chae HJ, Kim EY. (2001). Synthesis and characterization of a

novel extracellular polysaccharide by Rhodotorula glutinis. Applied Biochemistry and Biotechnology Vol. 95: 183-19.

Clarck S, Costello M, Drake M, Bobyfelt F. (2009). Sensory Evaluation of Dairy Products. 2nd ed. USA. Springer Science LLC. 271-333 pp.

Crescenzi V. (1995). Microbial polysaccharides of applied interest: ongoing research activities in Europe. Biotechnol Prog 11(3):251-9.

Cui F, Zhao L. (2012). Optimization of xylanase production from Penicillium spwx-z1 by a two-step statistical strategy: Plackett-Burman and Box-Behnken experimental design. International Journal of Molecular Science 13(8):10630-46.

Da Silva WJ, Gonc¸alves LM, Seneviratne J, Parahitiyawa N, Samaranayake LP, Del Bel Cury, AA. (2012). Exopolysaccharide matrix of developed Candida albicans biofilms after exposure to antifungal agents. Brazilian Dentistry Journal 23(6):716-722.

De Almeda GMD, Costa SF, Melhem M, Mottaal, Szeszs MW, Miyashitaf, Pierrotti LC, Rossi F, Burattini MN. (2008). Rhodotorula spp. isolated from blood cultures: clinical and microbiological aspects. Medical Mycology, 46:547-556.

Ghada S, Manal I, Mahmoud G, Asker M, Ghazy, EA. (2012). Production and biological evaluation of exopolysaccharide from isolated Rhodotorula glutinins. Australian Journal of Basic and Applied Sciences 6(3): 401-408.

Harris RA, Crabb DW. (2010). Metabolic Interrelationships: In Textbooks of Biochemistry with clinical correlations. 7th edition (Thomas M. Devlin editor). John Wiley and Sons, Inc. USA 1240 p.

Helferich W, Winter CK. (2001). Food Toxicology. USA: CRC Press LLC. 224 p.

Hwang HJ, Kim SW, Lim JM, Kim HO, Kim HM, Yun JW. (2005). Hypoglycemic effect of crude exopolysaccharide produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats. Life

Science 76(2005)3069-3080.

Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S. (2011). Acute oral toxicity of methanolic seed extract of Cassia fistula in mice. Molecules16:5268–5282.

Khan CM, Line S, Allen DG, Constable PD, Davies PD, Quensenberry KE, Reeves KE, Sharma JM, Smith RKW, Treadwell T. (2010). The Merck veterinary manual. 10th edition. Merck and Co., Inc New Jersey, USA.

Kim DH, Yang BK, Jeong SC, Hur NJ, Das S, Yun, JW, Choi JW, Lee YS, Song CH. (2001). A preliminary study on the hypoglycemic effect of the exopolymers produced by five different medicinal mushrooms. Journal of Microbiology and Biotechnology 11:167–171

Kirsten TB, Bernardi MM. (2010). Acute toxicity of Psilocybe cubensis (Ear.) Sing., and Strophariaceae, aqueous extract in mice. Review of Brazilian Farmacogenetics. 20:3

Lo HC, Tu ST, Lin KC, Lin SC. (2004). The anti-hyperglycemic activity of the fruiting body of Cordyceps in diabetic ratsinduced by nicotinamide and streptozotocin. Life Sciences 74, 2897–2908.

Majno G, Gabbiani G, Hirschel BJ, Ryan GB, Statkov P. (1971). Contraction of granulation tissue in vitro: Similarity to smooth muscle. Science 173 : 548–550.

Neidert KC, Dorner B. (2004). Nutrition care of the older adult. 2nd edition. Chicago, IL. American Diabetic Association 139p.

Ramirez, MAJ, Dizon EI, Mercado SM. (2015). Substrate Optimization for Exopolysaccharide Production by Rhodotorula minuta BIOTECH 2178 using Simplex-Lattice Design. Journal of Society and Technology, 5:34-41

Reginio Jr. FC. (2013). Quality and Safety Evaluation of Monascus Biopigment Beverage. [MS thesis]. College, Laguna, Philippines: University of the Philippines Los Ba˜nos. 137 p.(Available at the UPLB


Strutz F, Muller GA. (2006). Renal fibrosis and the origin of the renal fibroblast. Nephrol Dial Transplant 46:126-130.

T´urmina JA, Carraro E, Alves DA, Cunha MA, Dekker RF, Barbosa AM, Dos Santos FS, Silva LA, Malfatti CM. (2012). Toxicological Assessment of -(16)-Glucan (Lasiodiplodan) in Mice during a 28-day feeding study by gavage. Molecules. 17:14298-1430

Wirth F, Goldani LZ. (2012). Experimental Rhodotorulosis infection in rats. APMIS. 20(3):231-235


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