Preliminary Findings on Supplementation of Snapper Fry, Lutjanus sp. with DHA-rich Thraustochytrid Strain, Schizochytrium sp. LEY7 Showed Increased Growth, Survival and Robustness

Gladys Ludevese-Pascual


In this study, a preliminary experiment was conducted to investigate the beneficial effects of an indigenous DHA-rich thraustochytrid strain, Schizochytrium sp. LEY7 as supplement for snapper fry Lutjanus sp. using a long-term enrichment method (24 h) via Artemia. Results on the average total length (ATL) indicated that Schizohchytrium sp. LEY7 can be used as a growth enhancer (3.07 ± 0.03 mm) over the hatchery-produced emulsion (2.87 ± 0.04 mm) and the commercial enrichment SELCO (2.91 ± 0.04 mm). Starved treatment has an ATL of 2.89 ± 0.03 at the end of the feeding trial. Highest survival was obtained in the treatment fed Schizochytrium-enriched Artemia, with a mean survival of 65.52 ± 4.83, as compared to treatments fed Artemia enriched either with hatchery-produced emulsion (30.69 ± 25.17) or SELCO (54.14 ± 4.48). Survival of the starved treatment was 27.93 ± 10.69. There was a trend of lowest mortality seen for the treatment fed Schizochytrium-enriched Artemia when immersed at different salinity levels. Treatment fed SELCO-enriched Artemia was infested with the parasitic dinoflagellate Amyloodinium sp. Overall, the preliminary data obtained from this study indicate that the supplementation of Schizochytrium sp. LEY7 increases the quality of snapper fry and its chance of survival under adverse environmental conditions. Confirmatory experiments are therefore suggested to verify the results obtained.


Enrichment; Docosahexaenoic acid (DHA); Salinity stress test; Artemia sp.


Arafiles, K.H., Alcantara, J.C.O., Batoon, J.A.L., Galura, F.S., Cordero, P., Leano, ˜E., & Dedeles, G. (2011). Cultural optimization of thraustochytrids for biomass and fatty acid production. Mycosphere-Journal of Fungal Biology, 2, 521-531.

Copeman, L.A., Parrish, C.C., Brown, J.A., Harel, M. (2002). Effects of

docosahexaenoic, eicosapentaenoic and arachidonic acids on the early growth, survival, lipid composition and pigmentation of yellowtail flounder (Limanda ferruginea): A live food enrichment experiment. Aquaculture, 210, 285–304.

Cruz-Lacierda, E.R., Maeno, Y., Pineda, A.J.T., & Matey, V.E. (2004). Mass mortality of hatchery-reared milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus) caused by Amyloodinium ocellatum (Dinoflagellida). Aquaculture, 236(1-4),85-94.

Dhert, P., Lavens, P., & Sorgeloos, P. (1992). A simple test for quality evaluation of cultured fry of marine fish. Med. Fac.Landbouww. University of Gent.FAO. (2000). The state of world fisheries and

aquaculture 2000. Rome, Italy: FAO,FAO. (2009). The state of the world fisheries and aquaculture 2008. Retrieved from

FAO, (2014). The state of world fisheries and aquaculture. Rome, Italy: FAO Glencross, B.D., & Smith, D.M. (1999) The dietary linoleic and linolenic fatty acids requirements of the prawn, Penaeus monodon.Aquaculture Nutrition, 5, 53–63.

Gonzalez-F ´ elix, M.L. & Perez-Velazquez, M. ´(2002). Current status of lipid nutrition of Pacific white shrimp Litopenaeus vannamei. In L.E. Cruz–Surez, D. Ricque–Marie, M. Tapia–Salazar, M.G. Gaxiola-Cortes & N. Simoes N. (Eds.), Advances on nutricion acuicola VI.

Memorias del VI Simposium Internationalde Nutricion Acuicola (pp. 35–45).

Cancun, Quitana Roo, Mexico. Kamlangdee, N. & Fan, K.W. (2003).

Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove. Songklanakarin Journal of Science and Technology, 25, 643–650.

Kolakowska, A., Olley, J. & Dunstan, G.A. (2002). Fish lipids. In Z.E. Sikorski & A Kolakowska (Eds.) Chemical and functional properties of food lipids (pp.221–264). FL, USA: CRC Press

Leano, E., Gapasin, R., Polohan, B., & ˜Vrijmoed, L. (2003). Growth and fatty acid production of thraustochytrids from Panay mangroves, Philippines. Fungal Diversity, 12, 111–122.

Lee, D.J., Roehm, J.N., Yu, T.C. & Sinnhuber, R.O. (1967). Effect of omega-3 fatty acids on the growth rate of rainbow trout, Salmo

gairdnerii. Journal of Nutrition, 92. 93-8.

Lewis, T., Nichols, P. & McMeekin, T. (2000). Production of polyunsaturated fatty acids by Australian thraustochytrids: Aquaculture applications. Hatchery Feeds: Proceedings of a workshop held in Cairns, 9-10, 43–49.

Ludevese-Pascua,l G., Dela Pena, M. & ˜Tornalejo, J. (2014). Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, Schizochytrium sp. LEY7 using low-cost substrates at optimum culture conditions. Aquaculture Research, 47, 318–328.

Marques, A., Dinh, T., Ioakeimidis, C., Huys, G., Swings, J., Verstraete, W., Dhont, J., Sorgeloos, P. & Bossier, P. (2005). Effects of bacteria on Artemia franciscana cultured in different gnotobiotic environments. Applied and Environmental Microbiology, 71,(80), 4

–4 317.

Naylor, R., Goldberg, R., Primavera, J., Kautsky, N., Beveridge, M., Clay, J., Folke, C., Lubchenco, J., Mooney, H., & Troell, M. (2000). Effect of aquaculture on world fish supplies. Nature, 405, 1017-1024.

Nonwachai, T., Purivirojkul, W., Chuchird, N. & Limsuwan, C. (2009). Replacement of fish oil and fish meal with Schizochytrium sp. oil and soy bean meal in Pacific white shrimp (Litopenaeus vannamei) diets.

Kasetsart University Fisheries Research Bulletin 33:1–11.

Raghukumar, S. (2008) Thraustochytrid marine protists: production of PUFAs and other emerging technologies. Marine Biotechnology, 10, 631–640.

Rosario, W.R. (2008). The Philippines. In A. Lovatelli, M.J. Phillips, J.R. Arthur & K. Yamamoto (Eds). FAO/NACA regional workshop on the future of mariculture:A regional approach for responsible development in the Asia-Pacific region. Guangzhou, China: FAO Fisheries Proceedings.

Ruyter, B., Røsjø, C., Einen, O. & Thomassen, M.S. (2000). Essential fatty acids in Atlantic salmon: Effects of increasing dietary doses of n-6 and n-3 fatty acids on growth, survival and fatty acid composition

of liver, blood and carcass. Aquaculture Nutrition, 6, 119-127.

Thompson, J.M., & Bergersen, E.P. (1991). Role of size, conditioning and lipid content in the overwinter survival of age-0 Colorado squawfish. Transactions of the American Fisheries Society, 120, 346–353.

Turchini, G.M., Torstensen, B.E., & Ng, W.K. (2009). Fish oil replacement in finfish nutrition.Reviews in Aquaculture, 1, 10–57.

United Nations, (2010). World population prospects: The 2010 revision. New York:United Nations; 2011. Retrieved from

Wouters, R., Van Hauwaert, A., Naessens, E., Ramos, X., Pedrazzoli, A., & Lavens, P. (1997). The effect of dietary n-3 HUFA and 22:6n-320:5n-3 ratio on white shrimp larvae and postlarvae. Aquaculture

International, 5, 113–126.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.